This pape
r summa
rizes the
recent activities of the Ch
romosome-Cent
ric Human P
roteome P
roject (C-HPP) conso
rtium, which develops new technologies to identify yet-to-be annotated p
roteins (te
rmed 鈥渕issing p
roteins鈥? in biological samples that lack sufficient expe
rimental evidence at the p
rotein level fo
r confident p
rotein identification. The C-HPP also aims to identify new p
rotein fo
rms that may be caused by genetic va
riability, post-t
ranslational modifications, and alte
rnative splicing. P
roteogenomic data integ
ration fo
rms the basis of the C-HPP鈥檚 activities; the
refo
re, we have summa
rized some of the key app
roaches and thei
r roles in the p
roject. We p
resent new analytical technologies that imp
rove the chemical space and lowe
r detection limits coupled to bioinfo
rmatics tools and some publicly available
resou
rces that can be used to imp
rove data analysis o
r suppo
rt the development of analytical assays. Most of this pape
r鈥檚 content has been compiled f
rom poste
rs, slides, and discussions p
resented in the se
ries of C-HPP wo
rkshops held du
ring 2014. All data (poste
rs, p
resentations) used a
re available at the C-HPP Wiki (
ref="http://c-hpp.webhosting.rug.nl/" class="extLink">http://c-hpp.webhosting.rug.nl/) and in the Supporting Information.