Bifunctional Dendronized Cellulose Surfaces as Biosensors
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- 作者：Maria I. Montan虄ez ; Yvonne Hed ; Simon Utsel ; Jarmo Ropponen ; Eva Malmstro虉m ; Lars Wa虋gberg ; Anders Hult ; Michael Malkoch
- 刊名：Biomacromolecules
- 出版年：2011
- 出版时间：June 13, 2011
- 年：2011
- 卷：12
- 期：6
- 页码：2114-2125
- 全文大小：1231K
- 年卷期：v.12,no.6(June 13, 2011)
- ISSN：1526-4602
文摘
Well-defined dendronized cellulose substrates displaying multiple representations of dual-functionality were constructed by taking advantage of the efficiency of the click reaction combined with traditional anhydride chemistry. First, activated cellulose surfaces were decorated with several generations of dendrons, and their peripheral reactive groups were subsequently reacted with a trifunctional orthogonal monomer. The generated substrate tool box was successfully explored by accurately tuning the surface function using a versatile orthogonal dual postfunctionalization approach. In general, the reactions were monitored by using a click-dye reagent or a quartz crystal microbalance (QCM) technique, and the resulting surfaces were well-characterized using XPS, FT-IR, and contact angle measurements. Utilizing this approach two different surfaces have been obtained; that is, triethylenglycol oligomers and amoxicillin molecules were efficiently introduced to the dendritic surface. As a second example, mannose-decorated hydroxyl functional surfaces illustrated their potential as biosensors by multivalent detection of lectin protein at concentration as low as 5 nM.