Antibody Modified Porous Silicon Microparticles for the Selective Capture of Cells

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• 作者：Bin Guan ; Astrid Magenau ; Simone Ciampi ; Katharina Gaus ; Peter J. Reece ; J. Justin Gooding
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：July 16, 2014
• 年：2014
• 卷：25
• 期：7
• 页码：1282-1289
• 全文大小：391K
• ISSN：1520-4812

文摘

Herein, the ability of porous silicon (PSi) particles for selectively binding to specific cells is investigated. PSi microparticles with a high reflectance band in the reflectivity profile are fabricated, and subsequently passivated and modified with antibodies via the Cu(I)-catalyzed alkyne鈥揳zide cycloaddition reaction and succimidyl activation. To demonstrate the ability of the antibody-modified PSi particles to selectively bind to one cell type over others, HeLa cells were transfected with surface epitopes fused to fluorescent proteins. The antibody-functionalized PSi particles showed good selectivity for the corresponding surface protein on HeLa cells, with no significant cross-reactivity. The results are important for the application of PSi particles in cell sensing and drug delivery.