Polymerization of Unprotected Synthetic Peptides: A View toward Synthetic Peptide Vaccines
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- 作者：Neil M. O'Brien-Simpson ; Nicholas J. Ede ; Lorena E. Brown ; John Swan ; and David C. Jackson
- 刊名：Journal of the American Chemical Society
- 出版年：1997
- 出版时间：February 12, 1997
- 年：1997
- 卷：119
- 期：6
- 页码：1183 - 1188
- 全文大小：285K
- 年卷期：v.119,no.6(February 12, 1997)
- ISSN：1520-5126
文摘
A generic method is reported for the assembly of multi-peptidepolymers in which peptides are synthesizedin the solid phase, the N-terminal residue acryloylated, and thederivitized peptides cleaved, purified and finallypolymerized by free radical induced polymerization. The highmolecular weight polymers generated in this wayhave individual peptides pendant from a backbone support.Incorporation of 6-aminohexanoyl or other residue(s)atthe N-terminus of the peptide prior to acryloylation allows the peptideto be distanced from the polymer backboneand incorporation of acryloylated reagents into the polymerizationmixture also permits distancing of pendant peptidesalong the length of the backbone support. The polymerizationprocess results in highly antigenic artificial proteinsas measured by ELISA. Because this approach allows theincorporation of the same or combinations of differentpurified peptides into polymers, it lends itself to the assembly ofpotential vaccine candidates containing epitopesfrom single or multiple pathogens into a single covalentstructure.