Radial Capillary Array Electrophoresis Microplate and Scanner for High-Performance Nucleic Acid Analysis

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• 作者：Yining Shi ; Peter C. Simpson ; James R. Scherer ; David Wexler ; Christine Skibola ; Martyn T. Smith ; and Richard A. Mathies
• 刊名：Analytical Chemistry
• 出版年：1999
• 出版时间：December 1, 1999
• 年：1999
• 卷：71
• 期：23
• 页码：5354 - 5361
• 全文大小：380K
• 年卷期：v.71,no.23(December 1, 1999)
• ISSN：1520-6882

文摘

The design, fabrication, and operation of a radial capillaryarray electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplateconsists of a central common anode reservoir coupled to96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detectionchannels. Loading of 96 samples in parallel is achievedusing a pressurized capillary array system. High-qualityseparations of 96 pBR322 restriction digest samples areachieved in <120 s with the microplate system. Thepractical utility and multicolor detection capability isdemonstrated by analyzing 96 methylenetetrahydrofolatereductase (MTHFR) alleles in parallel using a noncovalent2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.