The interest in the use of chelates for enhancing metaluptake by plants during phytoremediation and their widespreaduse in plant nutrient research requires that an easy,reproducible method be developed for chelate detectionin a variety of systems. This work examined the use of capillaryelectrophoresis for chelate analysis. Electropherogramsfor nitrilotriacetic acid (NTA) and ethylenediaminetetraaceticacid (EDTA) and their metal complexes were obtainedby capillary electrophoresis in a phosphate buffer by directUV detection at 185 nm. The metals used were Ca(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), Pb(II), and Fe(III). For thema
jority of metals studied, linear calibration curves wereobtained in the concentration range of 10-1000
M. Thedirect method of determination at 185 nm was found togive a higher detection sensitivity than direct detection at254 nm. Limits of detection for the metal chelate complexeswere in the range of 2-50
M. The analysis was fast (<6min), exhibited low relative standard deviations forretention time (<1%) and peak corrected area (<5%),and required no complex sample pretreatment. The methodwas used to demonstrate speciation in complex nutrientmedia.