pH-Driven Self-Assembly of Acidic Microbial Glycolipids
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- 作者：Niki Baccile ; Mohamed Selmane ; Patrick Le Griel ; Sylvain Prévost ; Javier Perez ; Christian V. Stevens ; Elisabeth Delbeke ; Susanne Zibek ; Michael Guenther ; Wim Soetaert ; Inge N. A. Van Bogaert ; Sophie Roelants
- 刊名：Langmuir
- 出版年：2016
- 出版时间：June 28, 2016
- 年：2016
- 卷：32
- 期：25
- 页码：6343-6359
- 全文大小：1038K
- 年卷期：0
- ISSN：1520-5827
文摘
Microbial glycolipids are a class of well-known compounds, but their self-assembly behavior is still not well understood. While the free carboxylic acid end group makes some of them interesting stimuli-responsive compounds, the sugar hydrophilic group and the nature of the fatty acid chain make the understanding of their self-assembly behavior in water not easy and highly unpredictable. Using cryo-transmission electron microscopy (cryo-TEM) and both pH-dependent in situ and ex situ small angle X-ray scattering (SAXS), we demonstrate that the aqueous self-assembly at room temperature (RT) of a family of β-d-glucose microbial glycolipids bearing a saturated and monounsaturated C18 fatty acid chain cannot be explained on the simple basis of the well-known packing parameter. Using the “pH-jump” process, we find that the molecules bearing a monosaturated fatty acid forms vesicles below pH 6.2, as expected, but the derivative with a saturated fatty acid forms infinite bilayer sheets below pH 7.8, instead of vesicles. We show that this behavior can be explained on the different bilayer membrane elasticity as a function of temperature. Membranes are either flexible or stiff for experiments performed at a temperature respectively above or below the typical melting point, TM, of the lipidic part of each compound. Finally, we also show that the disaccharide-containing acidic cellobioselipid forms a majority of chiral fibers, instead of the expected micelles.