Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids
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- 作者：Karin A. Zemski BerryRobert M. Barkley ; Joseph J. BerryJoseph A. Hankin ; Emmy Hoyes ; Jeffery M. Brown ; Robert C. Murphy
- 刊名：Analytical Chemistry
- 出版年：2017
- 出版时间：January 3, 2017
- 年：2017
- 卷：89
- 期：1
- 页码：916-921
- 全文大小：471K
- ISSN：1520-6882
文摘
Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.