Differential Protein Levels and Post-Translational Modifications in Spinal Cord Injury of the Rat

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• 作者：Leila Afjehi-Sadat ; Mika Brejnikow ; Sung Ung Kang ; Vinay Vishwanath ; Nadja Walder ; Kurt Herkner ; Heinz Redl ; Gert Lubec
• 刊名：Journal of Proteome Research
• 出版年：2010
• 出版时间：March 5, 2010
• 年：2010
• 卷：9
• 期：3
• 页码：1591-1597
• 全文大小：174K
• 年卷期：v.9,no.3(March 5, 2010)
• ISSN：1535-3907

文摘

Although changes in protein expression in spinal cord injury (SCI) would be of pivotal interest, information so far is limited. It was therefore the aim of the study to determine protein levels and post-translational modifications in the early phase following SCI in the rat. SCI was induced in Sprague−Dawley rats and sham operated rats served as controls. A gel-based proteomic approach using two-dimensional gel electrophoresis followed by quantification with specific software and subsequent identification of differentially expressed proteins by nano-ESI-LC-MS/MS was applied. Proteins of several pathways and cascades were dysregulated in SCI: 14-3-3 epsilon protein, dynein light chain 1, and tubulin beta-5 chain showed higher levels in SCI, whereas adenylyl cyclase associated protein 1, dihydropyrimidinase-related protein 2, F-actin capping protein subunit beta, glyceraldehyde-3-phosphate dehydrogenase, stress-induced phosphoprotein 1 and transthyretin showed lower levels in the injured tissue. Post-translational modifications indicated free oxygen radical attack on proteins in SCI. The occurrence of stress is indicated by deranged stress-induced phosphoprotein 1 and signaling abnormalities are reflected by adenylyl cyclase-associated protein 1 and 14-3-3 epsilon protein. The findings propose the involvement of the corresponding cascades and challenge further work into aberrant signaling and oxidative stress in SCI, which may form the basis for experimental intervention for spinal cord trauma.