Controlled Release of Chol-TEG-DNA from Nano- and Micropatterned SU-8 Surfaces by a Spreading Lipid Film

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• 作者：Yavuz Erkan ; Kiona Halma ; Ilja Czolkos ; Aldo Jesorka ; Paul Dommersnes ; Ravindra Kumar ; Tom Brown ; Owe Orwar
• 刊名：Nano Letters
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：8
• 期：1
• 页码：227 - 231
• 全文大小：511K
• 年卷期：v.8,no.1(January 2008)
• ISSN：1530-6992

文摘

We report the controlled release of immobilized cholesteryl-tetraethyleneglycol-DNA (chol-DNA) from micropatterned SU-8 surfaces by a spreadinglipid film. The release of chol-DNA is rapid and on the order of the spreading rate of the lipid film ages/gifchars/beta2.gif" BORDER=0 ALIGN="middle"> = 1-3 ages/entities/mgr.gif">m²/s (~10⁵ molecules of DNAper second). The lipid film serves as a poor solvent for the DNA adduct, which upon contact redistributes into the aqueous phase. Thus, therelease of DNA is accompanied by a change in surface hydrophobicity. The method can be used for creating arbitrary concentration profilesof DNA in solution over time or to dynamically change surface properties on demand in, for example, micro- and nanofluidic devices. Examplesof DNA release from spiral, comb, meander, and triangular as well as from nanoscale SU-8 lanes are shown.