Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors

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• 作者：A. S. De Le贸n ; T. Garnier ; L. Jierry ; F. Boulmedais ; A. Mu帽oz-Bonilla ; J. Rodr铆guez-Hern谩ndez
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：June 10, 2015
• 年：2015
• 卷：7
• 期：22
• 页码：12210-12219
• 全文大小：532K
• ISSN：1944-8252

文摘

Herein, we report the fabrication of microstructured porous surfaces with controlled enzymatic activity by combining the breath figures and the layer-by-layer techniques. Two different types of porous surfaces were designed based on fluorinated and carboxylated copolymers in combination with PS, using poly(2,3,4,5,6-pentafluorostyrene)-b-polystyrene (PS5F₃₁-b-PS₂₁) and polystyrene-b-poly(acrylic acid) (PS₁₉-b-PAA₁₀) block copolymers, respectively. For comparative purposes, flat surfaces having similar chemistry were obtained by spin-coating. Poly(sodium 4-styrenesulfonate)/poly(allylamine hydrochloride) (PSS/PAH) multilayers incorporating alkaline phosphatase (ALP) were built on these porous surfaces to localize the enzyme both inside and outside of the pores using PS/PS5F₃₁-b-PS₂₁ surfaces and only inside the pores on PS/PS₁₉-b-PAA₁₀ surfaces. A higher catalytic activity of ALP (about three times) was obtained with porous surfaces compared to the flat ones. The catalysis happens specifically inside the holes of PS/PS₁₉-b-PAA₁₀surfaces, where ALP is located. This opens the route for applications in microreactors.