文摘
In this study, we addressed the question of whether anomic approach could genuinely be useful for biomarkerprofile analysis across different field sites with differentphysicochemical characteristics. We collected earthworms(Lumbricus rubellus) from seven sites with very differentlevels of metal contamination and prevailing soil type andanalyzed tissue extracts by 1H nuclear magnetic resonancespectroscopy. Pattern recognition analysis of the data showedthat both site- and contaminant-specific effects on themetabolic profiles could be discerned. Zinc was identifiedas the probable major contaminant causing a metabolicchange in the earthworms. Individual sites could be resolvedon the basis of NMR spectral profiles by principalcomponent analysis; these site differences may also havebeen caused by additional abiotic factors such as soilpH. Despite an inevitable degree of confounding betweensite and contaminant concentrations, it was possible toidentify metabolites which were correlated with zinc acrossall different sites. This study therefore acts as a proof ofprinciple for the use of NMR-based metabolic profiling as adiagnostic tool for ecotoxicological research in pollutedfield soils.