Selective Naked-Eye Detection of Hg2+ through an Efficient Turn-On Photoinduced Electron Transfer Fluorescent Probe and Its Real Applications

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• 作者：Priyanka Srivastava ; Syed S. Razi ; Rashid Ali ; Ramesh C. Gupta ; Suresh S. Yadav ; Gopeshwar Narayan ; Arvind Misra
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：September 2, 2014
• 年：2014
• 卷：86
• 期：17
• 页码：8693-8699
• 全文大小：405K
• ISSN：1520-6882

文摘

A simple molecular fluorescent probe 5 has been designed and synthesized by appending anthracene and benzhydryl moieties through a piperazine bridge. The probe upon interaction with different metal ions showed high selectivity and sensitivity (2 ppb) for Hg²⁺ through fluorescence 鈥渢urn-on鈥?response in HEPES buffer. The significant fluorescence enhancement (鈭?0-fold) is attributable to PET arrest due to complexation with nitrogen atoms of the piperazine unit and Hg²⁺ in 1:2 stoichiometry, in which a naked-eye sensitive fluorescent blue color of solution changed to a blue-green (switched-on). As a proof of concept, promising prospects for application in environmental and biological sciences 5 have been utilized to detect Hg²⁺ sensitively in real samples, on cellulose paper strips, in protein medium (like BSA), and intracellularly in HeLa cells. Moreover, the optical behavior of 5 upon providing different chemical inputs has been utilized to construct individual logic gates and a reusable combinational logic circuit. The combinational circuit (switch ON mode; OR logic gate) is easily resettable to the original position (switch OFF mode; INHIBIT logic gate) by applying reset chemical inputs (OH^{鈥?/sup> and PO₄3鈥?/sup>) with great reproducibility.}