Protein engineering projects often amass numerous raw DNA sequences, but no readi
ly avai
lab
le software combines sequence processing and activity corre
lation required for efficient
lead identification. XLibraryDisp
lay is an open source program integrated into Microsoft Exce
l for Windows that automates batch sequence processing via a simp
le step-by-step, menu-driven graphica
l user interface. XLibraryDisp
lay accepts any DNA temp
late which is used as a basis for trimming, fi
ltering, trans
lating, and a
ligning hundreds to thousands of sequences (raw, FASTA, or Phred PHD fi
le formats). Key steps for
library characterization through
lead discovery are avai
lab
le inc
luding
library composition ana
lysis, fi
ltering by experimenta
l data, graphing and corre
lating to experimenta
l data, a
lignment to structura
l data extracted from PDB fi
les, and generation of PyMOL visua
lization scripts. Though
larger data sets can be hand
led, the program is best suited for ana
lyzing approximate
ly 10鈥?00 or fewer
leads or na茂ve c
lones which have been characterized using Sanger sequencing and other experimenta
l approaches. XLibraryDisp
lay can be down
loaded for free from
librarydisplay/" class="extLink">sourceforge.net/projects/xlibrarydisplay/.