Immunosorbents Coupled On-Line with Liquid Chromatography for the Determination of Fluoroquinolones in Chicken Liver

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• 作者：Carol K. Holtzapple ; Sandra A. Buckley ; and Larry H. Stanker
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1999
• 出版时间：July 1999
• 年：1999
• 卷：47
• 期：7
• 页码：2963 - 2968
• 全文大小：68K
• 年卷期：v.47,no.7(July 1999)
• ISSN：1520-5118

文摘

Four fluoroquinolones were analyzed in fortified chicken liver using an automated, on-lineimmunoaffinity extraction method. The fluoroquinolones were extracted from the liver matrix usingan immunoaffinity capture column containing anti-sarafloxacin antibodies covalently cross-linkedto protein G. After interfering liver matrix components had been washed away, the capturedfluoroquinolones were automatically eluted directly onto a reversed phase column. Liquidchromatographic analyses were performed by isocratic elution using 2% acetic acid/acetonitrile (85:15) as the mobile phase and an Inertsil phenyl column with fluorescence detection at excitationand emission wavelengths of 280 and 444 nm, respectively. No significant interferences from thesample matrix were observed, indicating good selectivity with the immunoaffinity column. Overallrecoveries from fortified liver samples (20, 50, and 100 ng/g) ranged between 85.7 and 93.5% withstandard deviations of <5%. The limit of quantification for each fluoroquinolone was 1 ng/mL. Thelimits of detection, based on a signal-to-noise ratio of 5:1, were 0.47, 0.32, 0.87, and 0.53 ng/mL forciprofloxacin, enrofloxacin, sarafloxacin, and difloxacin, respectively.Keywords: Fluoroquinolone; immunoaffinity chromatography; on-line detection method; bovinetissues; food safety