Analysis of Potato Glycoalkaloids by a New ELISA Kit

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• 作者：Mendel Friedman ; Felicidad F. Bautista ; Larry H. Stanker ; and Karen A. Larkin
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1998
• 出版时间：December 1998
• 年：1998
• 卷：46
• 期：12
• 页码：5097 - 5102
• 全文大小：74K
• 年卷期：v.46,no.12(December 1998)
• ISSN：1520-5118

文摘

Steroidal glycoalkaloids are naturally occurring compounds present in solanaceous plants includingpotatoes. They are reported to be toxic to animals and humans. The recognition of their potentialtoxicity has led to implementation of guidelines limiting glycoalkaloid content. The effectivenessof these guidelines is dependent upon reliable analytical methods for their analysis. The objectiveof this study was to develop a simple, rapid, and inexpensive immunoassay for potato glycoalkaloidsthat correlates with HPLC. This was successfully demonstrated with various potato samples,including eight fresh potato varieties; potato flesh, peel, sprouts, and leaves; and processed productssuch as French fries, chips, and skins. Storage of the ELISA kit in a refrigerator for >3 monthsdid not affect its effectiveness. The use of a stable, accurate, and highly sensitive ELISA kit shouldfacilitate (a) development of standard protocols for handling and sampling of potatoes to minimizepre- and postharvest glycoalkaloid formation; (b) analysis of foliar glycoalkaloids, thus saving plantbreeders considerable time, effort, and cost; (c) marketing potatoes at lower cost; (d) measurementof the metabolism and distribution of glycoalkaloids in animals and humans; and (e) assurance tothe consumer of eating a good quality potato.Keywords: hars/alpha.gif" BORDER=0>-Chaconine; ELISA kit; HPLC; potatoes; potato chips; potato fries; potato leaves; potatopeel; potato sprouts; hars/alpha.gif" BORDER=0>-solanine