Development of a Monoclonal Antibody-Based cELISA for the Analysis of Sulfadimethoxine. 1. Development and Characterization of Monoclonal Antibodies and Molecular Modeling Studies of Antibody Recognit

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• 作者：Mark T. Muldoon ; Carol K. Holtzapple ; Sudhir S. Deshpande ; Ross C. Beier ; and Larry H. Stanker
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2000
• 出版时间：February 2000
• 年：2000
• 卷：48
• 期：2
• 页码：537 - 544
• 全文大小：512K
• 年卷期：v.48,no.2(February 2000)
• ISSN：1520-5118

文摘

Sulfonamide antibiotics are used to treat a variety of bacterial and protozoan infections in cattle,swine, and poultry. Current residue methods for the analysis of sulfonamides in animal-based foodproducts include bioassays, chromatographic methods (HPLC, GLC), and immunoassays. Mostimmunoassays have employed highly specific polyclonal antibodies. In this paper, we describe theisolation of monoclonal antibodies against sulfadimethoxine (SDM) that vary in their sensitivitiesand cross-reactivities against a large number of sulfonamides. The most sensitive monoclonalantibody, designated SDM-18, exhibits an IC₅₀ value for SDM of 1.53 ppb. Another monoclonalantibody, designated SDM-44, exhibits IC₅₀ values for six sulfonamides well below the establishedthreshold level of 100 ppb for animal tissues. Molecular modeling studies of the cross-reactive drugssuggest that, depending on the monoclonal antibody, both steric and electronic features governantibody binding. Due to the diversity of these monoclonal antibodies, it should be possible to designboth compound- and class-specific monoclonal antibody-based immunoassays.Keywords: Sulfadimethoxine; sulfonamide; immunoassay; molecular modeling