UPLC-ESI-TOF MS-Based Metabolite Profiling of the Antioxidative Food Supplement Garcinia buchananii

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• 作者：Timo D. Stark ; Sofie L枚sch ; Junichiro Wakamatsu ; Onesmo B. Balemba ; Oliver Frank ; Thomas Hofmann
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：63
• 期：32
• 页码：7169-7179
• 全文大小：651K
• ISSN：1520-5118

文摘

Comparative antioxidative analyses of aqueous ethanolic extracts from leaf, root, and stem of Garcinia buchananii revealed high activity of all three organs. To investigate the metabolite composition of the different parts of G. buchananii, an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectra (MS^e) was performed. Unsupervised statistics (PCA) highlighted clear differences in the metabolomes of the three organs. OPLS-DA revealed (2R,3S,2鈥?i>R,3鈥?i>R)-GB-1, (2R,3S)-morelloflavone, and (2R,3S)-volkensiflavone as the most decisive marker compounds discriminating leaf from root and stem extract. Leaves represent the best source to isolate GB-1, morelloflavone, and volkensiflavone. Root extract is the best organ to isolate xanthones and stem bark extract the best source to isolate (2R,3S,2鈥?i>R,3鈥?i>R)-manniflavanone; the identified polyisoprenylated benzophenones are characteristic compounds for the leaf organ. Morelloflavone, volkensiflavone, and garcicowin C were isolated for the first time from G. buchananii, identified via MS, NMR, and CD spectroscopy, and showed in H₂O₂ scavenging, H/L-TEAC, and H/L-ORAC assays moderate to strong in vitro antioxidative activities.