IR-MALDI-MS Analysis of HPTLC-Separated Phospholipid Mixtures Directly from the TLC Plate
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- 作者：Andreas Rohlfing ; Johannes Mü ; thing ; Gottfried Pohlentz ; Ute Distler ; Jasna Peter-Katalini
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- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：August 1, 2007
- 年：2007
- 卷：79
- 期：15
- 页码：5793 - 5808
- 全文大小：4587K
- 年卷期：v.79,no.15(August 1, 2007)
- ISSN：1520-6882
文摘
The application of a recently developed direct couplingof high-performance thin-layer chromatography (HPTLC)and infrared matrix-assisted laser desorption/ionizationorthogonal extracting time-of-flight mass spectrometry(Dreisewerd, K.; Müthing, J.; Rohlfing, A.; Meisen, I.;Vukelic, Z.; Peter-Katalinic, J.; Hillenkamp, F.; Berkenkamp, S. Anal. Chem. 2005, 77, 4098-4107) to theanalysis of phospholipid mixtures is demonstrated. Mixtures of six phospholipid types were exemplarily analyzed.The sensitivity was found to be in the range between about10 and 150 pmol of material spotted for HPTLC, depending on phospholipid acidity, Rf value, and ion polarity.The lateral resolution of the analysis is on the order ofthe laser focus diameter of about 220 × 300 
m2,allowing differentiation between phospholipid species ofdifferent acyl chain composition within one single HPTLCband, which were undistiguishable by a mere visualassessment. Analyte diffusion due to the addition ofglycerol to the HPTLC plate was found to be-if at allnotable-of only minor importance.
m2,allowing differentiation between phospholipid species ofdifferent acyl chain composition within one single HPTLCband, which were undistiguishable by a mere visualassessment. Analyte diffusion due to the addition ofglycerol to the HPTLC plate was found to be-if at allnotable-of only minor importance.