Computational Studies of Epidermal Growth Factor Receptor: Docking Reliability, Three-Dimensional Quantitative Structure−Activity Relationship Analysis, and Virtual Screening Studies

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• 作者：Concettina La Motta ; Stefania Sartini ; Tiziano Tuccinardi* ; Erika Nerini ; Federico Da Settimo ; Adriano Martinelli
• 刊名：Journal of Medicinal Chemistry
• 出版年：2009
• 出版时间：February 26, 2009
• 年：2009
• 卷：52
• 期：4
• 页码：964-975
• 全文大小：438K
• 年卷期：v.52,no.4(February 26, 2009)
• ISSN：1520-4804

文摘

An aberrant activity of the epidermal growth factor receptor (EGFR) has been shown to be related to many human cancers, such as breast and liver cancers, thus making EGFR an attractive target for antitumor drug discovery. In this study we evaluated the reliability of various kinds of docking software and procedures to predict the binding disposition of EGFR inhibitors. By application of the best procedure and use of more than 200 compounds, a receptor-based 3D-QSAR model for EGFR inhibition was developed. On the basis of the results obtained, the possibility of developing virtual screening studies was also evaluated. The VS procedure that proved to be the most reliable from a computational point of view was then used to filter the Maybridge database in order to identify new EGFR inhibitors. Enzymatic assays revealed that among the eight top-scoring compounds, seven proved to inhibit EGFR activity at a concentration of 100 μM, two of them exhibiting IC₅₀ values in the low micromolar range and one in the nanomolar range. These results demonstrate the validity of the methodologies followed. Furthermore, the two low micromolar compounds may be considered as very interesting leads for the development of new EGFR inhibitors.