Tailor-Made Quantum Dot and Iron Oxide Based Contrast Agents for in Vitro and in Vivo Tumor Imaging
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- 作者：Elmar P枚selt ; Christian Schmidtke ; Steffen Fischer ; Kersten Peldschus ; Johannes Salamon ; Hauke Kloust ; Huong Tran ; Andrea Pietsch ; Markus Heine ; Gerhard Adam ; Udo Schumacher ; Christoph Wagener ; Stephan F枚rster ; Horst Weller
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：April 24, 2012
- 年：2012
- 卷：6
- 期：4
- 页码：3346-3355
- 全文大小：592K
- 年卷期：v.6,no.4(April 24, 2012)
- ISSN：1936-086X
文摘
The biofunctionalization of CdSe/CdS/ZnS quantum dots and Fe3O4 nanocrystals using a novel ligand system based on polyisoprene-block-poly(ethylene oxide) ligands is described. The synthesis includes a partial ligand exchange of the hydrophobic nanocrystals with amino-functionalized polyisoprene ligands, followed by seeded micelle formation of the diblock-copolymers in water. The resulting water-soluble quantum dots showed fluorescence quantum efficiencies in the 40 to 50% range and extraordinary fluorescence stability in the biological environment after cross-linking of the polyisoprene moiety of the ligand shell. No toxicity was detected by water-soluble tetrazolium (WST8) and lactate dehydrogenase (LDH) assays, even at very high nanoparticle concentrations, and almost no nonspecific cell adhesion was detected. The ligand shell was further coupled to the antigen-related cell adhesion molecule (CEACAM) specific monoclonal antibody T84.1. The so-conjugated Fe3O4 nanocrystals allowed in vitro and in vivo tumor targeting by magnetic resonance imaging.