Biosynthesis of Mupirocin by Pseudomonas fluorescens NCIMB 10586 Involves Parallel Pathways
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- 作者：Shu-Shan Gao ; Joanne Hothersall ; Ji鈥檈n Wu ; Annabel C. Murphy ; Zhongshu Song ; Elton R. Stephens ; Christopher M. Thomas ; Matthew P. Crump ; Russell J. Cox ; Thomas J. Simpson ; Christine L. Willis
- 刊名：Journal of the American Chemical Society
- 出版年：2014
- 出版时间：April 9, 2014
- 年：2014
- 卷：136
- 期：14
- 页码：5501-5507
- 全文大小：438K
- 年卷期：v.136,no.14(April 9, 2014)
- ISSN：1520-5126
文摘
Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.