Sample Filtration, Concentration, and Separation Integrated on Microfluidic Devices
详细信息 查看全文
- 作者：B. Scott Broyles ; Stephen C. Jacobson ; and J. Michael Ramsey
- 刊名：Analytical Chemistry
- 出版年：2003
- 出版时间：June 1, 2003
- 年：2003
- 卷：75
- 期：11
- 页码：2761 - 2767
- 全文大小：148K
- 年卷期：v.75,no.11(June 1, 2003)
- ISSN：1520-6882
文摘
Microfabricated devices integrating sample filtration, solid-phase extraction, and chromatographic separation withsolvent programming were demonstrated. Filtering of thesample was accomplished at the sample inlet with an arrayof seven channels each 1 
m deep and 18 m wide.Sample concentration and separation were performed onchannels 5 m deep and 25 m wide coated with a C18phase, and elution was achieved under isocratic, step, orlinear gradient conditions. For the solid-phase extraction,signal enhancement factors of 400 over a standard injection of 1.0 s were observed for a 320-s injection. Fourpolycyclic aromatic compounds were resolved by openchannel electrochromatography in under 50 s. Chipoperation was unaffected by the presence of the 5-msilica particles at the filter entrance.
m deep and 18 m wide.Sample concentration and separation were performed onchannels 5 m deep and 25 m wide coated with a C18phase, and elution was achieved under isocratic, step, orlinear gradient conditions. For the solid-phase extraction,signal enhancement factors of 400 over a standard injection of 1.0 s were observed for a 320-s injection. Fourpolycyclic aromatic compounds were resolved by openchannel electrochromatography in under 50 s. Chipoperation was unaffected by the presence of the 5-msilica particles at the filter entrance.