Floating Lipid Bilayers Deposited on Chemically Grafted Phosphatidylcholine Surfaces

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• 作者：Arwel V. Hughes ; Jonathan R. Howse ; Aleksandra Dabkowska ; Richard A. L. Jones ; M. Jayne Lawrence ; Stephen J. Roser
• 刊名：Langmuir
• 出版年：2008
• 出版时间：March 4, 2008
• 年：2008
• 卷：24
• 期：5
• 页码：1989 - 1999
• 全文大小：394K
• 年卷期：v.24,no.5(March 4, 2008)
• ISSN：1520-5827

文摘

Floating supported bilayers (FSBs) are new systems which have emerged over the past few years to producesupported membrane mimics, where the bilayers remain associated with the substrate, but are cushioned from thesubstrates constraining influence by a large hydration layer. In this paper we describe a new approach to fabricatingFSBs using a chemically grafted phospholipid layer as the support for the floating membrane. The grafted lipid layerwas produced using a Langmuir-Schaeffer transfer of acryloyl-functionalized lipid onto a pre-prepared substrate, withAIBN-induced cross-polymerization to permanently bind the lipids in place. A bilayer of DSPC was then depositedonto this grafted monolayer using a combination of Langmuir-Blodgett and Langmuir-Schaeffer transfer. Theresulting system was characterized by neutron reflection under two water contrasts, and we show that the new systemshows a hydrating layer of ~17.5 Å in the gel phase, which is comparable to previously described FSB systems. Weprovide evidence that the grafted substrate is reusable after cleaning and suggest that this greatly simplifies thefabrication and characterization of FSBs compared to previous methods.