Tumor Cell Detection by Mass Spectrometry Using Signal Ion Emission Reactive Release Amplification

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• 作者：Zane Baird ; Valentina Pirro ; Stephen Ayrton ; Adam Hollerbach ; Cathleen Hanau ; Karen Marfurt ; Mary Foltz ; R. Graham Cooks ; Michael Pugia
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：July 19, 2016
• 年：2016
• 卷：88
• 期：14
• 页码：6971-6975
• 全文大小：273K
• 年卷期：0
• ISSN：1520-6882

文摘

A method is presented for the detection of circulating tumor cells (CTC) using mass spectrometry (MS), through reporter-ion amplification. Particles functionalized with short-chain peptides are bound to cells through antibody–antigen interactions. Selective release and MS detection of peptides is shown to detect as few as 690 cells isolated from a 10 mL blood sample. Here we present proof-of-concept results that pave the way for further investigations.