MicroRNA Functionalized Microporous Titanium Oxide Surface by Lyophilization with Enhanced Osteogenic Activity
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- 作者：Kaimin Wu ; Wen Song ; Lingzhou Zhao ; Mengyuan Liu ; Jun Yan ; Morten 脴stergaard Andersen ; J酶rgen Kjems ; Shan Gao ; Yumei Zhang
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2013
- 出版时间：April 10, 2013
- 年：2013
- 卷：5
- 期：7
- 页码：2733-2744
- 全文大小：1007K
- 年卷期：v.5,no.7(April 10, 2013)
- ISSN：1944-8252
文摘
Developing biomedical titanium (Ti) implants with high osteogenic ability and consequent rigid osseointegration is a constant requirement from the clinic. In this study, we fabricate novel miRNA functionalized microporous Ti implants by lyophilizing miRNA lipoplexes onto a microporous titanium oxide surface formed by microarc oxidation (MAO). The microporous titanium oxide surface provides a larger surface area for miRNA loading and enables spatial retention of the miRNAs within the pores until cellular delivery. The loading of lipoplexes into the micropores on the MAO Ti surface is facilitated by the superhydrophilicity and Ti鈥揙H groups gathering of the MAO surface after UV irradiation followed by lyophilization. A high miRNA transfection efficiency was observed in mesenchymal stem cells (MSCs) seeded onto the miRNA functionalized surface with no apparent cytotoxicity. When functionalizing the Ti surface with miR-29b that enhances osteogenic activity and antimiR-138 that inhibits miR-138 inhibition of endogenous osteogenesis, clear stimulation of MSC osteogenic differentiation was observed, in terms of up-regulating osteogenic expression and enhancing alkaline phosphatase production, collagen secretion and ECM mineralization. The novel miRNA functionalized Ti implants with enhanced osteogenic activity promisingly lead to more rapid and robust osseointegration of a clinical bone implant interface. Our study implies that lyophilization may constitute a versatile method for miRNA loading to other biomaterials with the aim of controlling cellular function.