Analytical Performance of an Ultrasonic Particle Focusing Flow Cytometer

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• 作者：Gregory R. Goddard ; Claire K. Sanders ; John C. Martin ; Gregory Kaduchak ; Steven W. Graves
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：November 15, 2007
• 年：2007
• 卷：79
• 期：22
• 页码：8740 - 8746
• 全文大小：531K
• 年卷期：v.79,no.22(November 15, 2007)
• ISSN：1520-6882

文摘

Creation of inexpensive small-flow cytometers is importantfor applications ranging from disease diagnosis in resource-poor areas to use in distributed sensor networks. Inconventional-flow cytometers, hydrodynamics focus particles to the center of a flow stream for analysis, whichrequires sheath fluid that increases consumable use andwaste while dramatically reducing instrument portability.Here we have evaluated, using quantitative measurementsof fluorescent microspheres and cells, the performanceof a flow cytometer that uses acoustic energy to focusparticles to the center of a flow stream. This evaluationdemonstrated measurement precision for fluorescenceand side scatter CVs for alignment microspheres of 2.54%and 7.7%, respectively. Particles bearing 7 × 10³ fluorophores were well resolved in a background of 50 nMfree fluorophore. The lower limit of detection was determined to be about 650 fluorescein molecules. Analysisof Chinese hamster cells on the system demonstrated thatacoustic focusing had no effect on cellular viability. Theseresults indicate that the ultrasonic flow cytometer has thenecessary performance for most flow cytometry applications. Furthermore, through robust engineering approaches and the combination of acoustic focusing withlow-cost light sources, detectors, and data acquisitionsystems, it will be possible to achieve a low-cost, trulyportable flow cytometer.