Effect of Sterol Structure on the Physical Properties of 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Membranes Determined Using 2H Nuclear Magnetic Resonance

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• 作者：Mehran Shaghaghi ; Mei-Ting Chen ; Ya-Wei Hsueh ; Martin J. Zuckermann ; Jenifer L. Thewalt
• 刊名：Langmuir
• 出版年：2016
• 出版时间：August 2, 2016
• 年：2016
• 卷：32
• 期：30
• 页码：7654-7663
• 全文大小：456K
• 年卷期：0
• ISSN：1520-5827

文摘

The effect of a series of phytosterols on lipid chain ordering in 1-palmitoyl(²H₃₁)-2-oleoyl-sn-glycero-3-phosphocholine (POPC-d₃₁) multibilayer vesicles was examined by ²H NMR spectroscopy at 25 °C. These results, along with existing data for other sterols, indicate that the ordering power of sterols in POPC-d₃₁ depends on subtle aspects of sterol structure. Cholesterol, 7-dehydrocholesterol (7-DHC), campesterol, β-sitosterol, ergosterol, brassicasterol, and stigmasterol all increase the lipid chain order as sterol concentration is increased. However, saturation of the ordering occurs at different sterol concentrations for ergosterol (as previously reported), brassicasterol, β-sitosterol, and stigmasterol. Here our interest lies in finding which part of the sterol structure is responsible for the observed saturation of the palmitoyl chain order as a function of sterol concentration. In particular, we propose that the saturation of the ordering of POPC-d₃₁/brassicasterol and POPC-d₃₁/stigmasterol membranes at quite low sterol concentrations is due to the presence of a double bond at C22. We also discuss how the structural differences between the sterols affect their ability to intercalate between the POPC acyl chains. Furthermore, the effective solubility of sterols in POPC is discussed in relation to the dependence of maximum POPC-d₃₁ chain order vs sterol concentration.