Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

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• 作者：Carina Pareja-Rivera ; Mayra Cuéllar-Cruz ; Nuria Esturau-Escofet ; Nicola DemitriMaurizio Polentarutti ; Vivian Stojanoff ; Abel Moreno
• 刊名：Crystal Growth & Design
• 出版年：2017
• 出版时间：January 4, 2017
• 年：2017
• 卷：17
• 期：1
• 页码：135-145
• 全文大小：651K
• ISSN：1528-7505

文摘

In this contribution we use nonconventional methods that help to increase the success rate of a protein crystal growth, and consequently of structural projects using X-ray diffraction techniques. In order to achieve this purpose, this contribution presents new approaches involving more sophisticated techniques of protein crystallization, not just for growing protein crystals of different sizes by using electric fields, but also for controlling crystal size and orientation. This latter was possible through the use of magnetic fields that allow to obtain protein crystals suitable for both high-resolution X-ray and neutron diffraction crystallography where big crystals are required. This contribution discusses some pros, cons and realities of the role of electromagnetic fields in protein crystallization research, and their effect on protein crystal contacts. Additionally, we discuss the importance of room and low temperatures during data collection. Finally, we also discuss the effect of applying a rather strong magnetic field of 16.5 T, for shorts and long periods of time, on protein crystal growth, and on the 3D structure of two model proteins.