Urinary Excretion of Black Raspberry (Rubus occidentalis) Anthocyanins and Their Metabolites

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• 作者：Qingguo Tian ; M. Monica Giusti ; Gary D. Stoner ; and Steven J. Schwartz
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：February 22, 2006
• 年：2006
• 卷：54
• 期：4
• 页码：1467 - 1472
• 全文大小：109K
• 年卷期：v.54,no.4(February 22, 2006)
• ISSN：1520-5118

文摘

Anthocyanins are the most abundant phenolic compounds, widely distributed in fruits and vegetables,and exhibit potent antioxidant capacity. Humans ingest a significant amount of anthocyanins in thedaily diet. The objective of the current study was to examine human absorption and metabolism ofblack raspberry anthocyanins when administered at high doses (2.69 ± 0.085 g/day). Ten healthymen consumed 45 g of freeze-dried black raspberries daily for 1 week. Urine samples were collectedover a 12 h period in 4 h intervals at day 1 and day 7. Urinary anthocyanins were analyzed by high-performance liquid chromatography coupled to a photodiode array detector and a tandem massspectrometer using precursor ion and product ion analyses. Anthocyanins were excreted in intactforms and metabolized into methylated derivatives in human urine. The urinary excretion ofanthocyanins reached a maximum concentration (1091.8 ± 1081.3 pmol/L, n = 10) during the 4-8h period after black raspberry ingestion. As compared to the anthocyanin distribution in blackraspberries, urinary cyanidin 3-xylosylrutinoside was detected at a higher concentration than that ofcyanidin-3-rutinoside.Keywords: Anthocyanins; black raspberries; anthocyanin methylated derivatives; absorption; metabolism