Development of an Immunoassay for the Pyrethroid Insecticide Esfenvalerate
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- 作者：Guomin Shan ; Donald W. Stoutamire ; Ingrid Wengatz ; Shirley J. Gee ; and Bruce D. Hammock
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1999
- 出版时间：May 1999
- 年：1999
- 卷：47
- 期：5
- 页码：2145 - 2155
- 全文大小：159K
- 年卷期：v.47,no.5(May 1999)
- ISSN：1520-5118
文摘
A competitive enzyme-linked immunosorbent assay was developed for the detection of the pyrethroidinsecticide esfenvalerate. Two haptens containing amine or propanoic acid groups on the terminalaromatic ring of the fenvalerate molecule were synthesized and coupled to carrier proteins asimmunogens. Five antisera were produced and screened against eight different coating antigens.The assay that had the least interference and was the most sensitive for esfenvalerate was optimizedand characterized. The I50 for esfenvalerate was 30 ± 6.2 
g/L, and the lower detection limit (LDL)was 3.0 ± 1.8 g/L. The assay was very selective. Other pyrethroid analogues and esfenvaleratemetabolites tested did not cross-react significantly in this assay. To increase the sensitivity of theoverall method, a C18 sorbent-based solid-phase extraction (SPE) was used for water matrix. Withthis SPE step, the LDL of the overall method for esfenvalerate was 0.1 g/L in water samples.Keywords: Immunoassay; esfenvalerate; pyrethroids; solid-phase extraction; residue analysis
g/L, and the lower detection limit (LDL)was 3.0 ± 1.8 g/L. The assay was very selective. Other pyrethroid analogues and esfenvaleratemetabolites tested did not cross-react significantly in this assay. To increase the sensitivity of theoverall method, a C18 sorbent-based solid-phase extraction (SPE) was used for water matrix. Withthis SPE step, the LDL of the overall method for esfenvalerate was 0.1 g/L in water samples.Keywords: Immunoassay; esfenvalerate; pyrethroids; solid-phase extraction; residue analysis