Development of an Enzyme-Linked Immunosorbent Assay for the Detection of the Pyrethroid Insecticide Fenpropathrin
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- 作者：Ingrid Wengatz ; Donald W. Stoutamire ; Shirley J. Gee ; and Bruce D. Hammock
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1998
- 出版时间：June 1998
- 年：1998
- 卷：46
- 期：6
- 页码：2211 - 2221
- 全文大小：154K
- 年卷期：v.46,no.6(June 1998)
- ISSN：1520-5118
文摘
A competitive enzyme-linked immunosorbent assay (ELISA) was developedfor the quantitativedetection of fenpropathrin[(RS)-
-cyano-3-phenoxybenzyl-2,2,3,3-tetramethylcyclopropanecarboxylate]. Polyclonal antisera were isolated from rabbits immunizedwith two different fenpropathrinhapten conjugates. One hapten contained an amino function; theother contained a carboxyl groupfor conjugation to carrier proteins. Mollusk hemocyanins,thyroglobulin, and fetuin were used ascarrier proteins. The antisera varied greatly in their affinitiesfor fenpropathrin. A homologousassay system using the coating antigen format was the most sensitive.The IC50 for fenpropathrinwas 20 
g/L, and the lower detection limit was 2.5 g/L.Pyrethroids, such as phenothrin, permethrin,resmethrin, fenvalerate, deltamethrin, cyfluthrin, and cypermethrin,and the pyrethroid metabolites,3-phenoxybenzoic acid and fenpropathrin acid, did not cross-reactsignificantly in this assay. Tenpercent acetone or methanol and a pH of 4 were determined to be optimumassay conditions. Variouscationic, anionic, and nonionic detergents had no significant effect onthe assay.Keywords: Fenpropathrin; pyrethroid; ELISA; pesticide; enzyme immunoassay;cross-reactivity
-cyano-3-phenoxybenzyl-2,2,3,3-tetramethylcyclopropanecarboxylate]. Polyclonal antisera were isolated from rabbits immunizedwith two different fenpropathrinhapten conjugates. One hapten contained an amino function; theother contained a carboxyl groupfor conjugation to carrier proteins. Mollusk hemocyanins,thyroglobulin, and fetuin were used ascarrier proteins. The antisera varied greatly in their affinitiesfor fenpropathrin. A homologousassay system using the coating antigen format was the most sensitive.The IC50 for fenpropathrinwas 20 g/L, and the lower detection limit was 2.5 g/L.Pyrethroids, such as phenothrin, permethrin,resmethrin, fenvalerate, deltamethrin, cyfluthrin, and cypermethrin,and the pyrethroid metabolites,3-phenoxybenzoic acid and fenpropathrin acid, did not cross-reactsignificantly in this assay. Tenpercent acetone or methanol and a pH of 4 were determined to be optimumassay conditions. Variouscationic, anionic, and nonionic detergents had no significant effect onthe assay.Keywords: Fenpropathrin; pyrethroid; ELISA; pesticide; enzyme immunoassay;cross-reactivity