Single Site Discrimination of Cytosine, 5-Methylcytosine, and 5-Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes
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- 作者：Jean-Louis H. A. Duprey ; Gemma A. Bullen ; Zheng-yun Zhao ; Dario M. Bassani ; Anna F. A. Peacock ; John Wilkie ; James H. R. Tucker
- 刊名：ACS Chemical Biology
- 出版年：2016
- 出版时间：March 18, 2016
- 年：2016
- 卷：11
- 期：3
- 页码：717-721
- 全文大小：287K
- ISSN：1554-8937
文摘
The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.