Spirolides Isolated from Danish Strains of the Toxigenic Dinoflagellate Alexandrium ostenfeldii
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- 作者：Shawna L. MacKinnon ; John A. Walter ; Michael A. Quilliam ; Allan D. Cembella ; Patricia LeBlanc ; Ian W. Burton ; William R. Hardstaff ; Nancy I. Lewis
- 刊名：Journal of Natural Products
- 出版年：2006
- 出版时间：July 2006
- 年：2006
- 卷：69
- 期：7
- 页码：983 - 987
- 全文大小：79K
- 年卷期：v.69,no.7(July 2006)
- ISSN：1520-6025
文摘
Using LC/MS methodology, spirolides were detected in two clonal isolates of Alexandrium ostenfeldii isolated fromLimfjorden, Denmark. Examination of the LC/MS profiles of extracts from these Danish cultures revealed the presenceof two dominant peaks representing two previously unidentified spirolide components and one minor peak identified asthe previously reported desmethyl spirolide C (1). Culturing of these clonal strains, LF 37 and LF 38, of A. ostenfeldiiresulted in the accumulation of sufficient cell biomass to allow for the isolation and structure elucidation of two newspirolides, 13,19-didesmethylspirolide C (2) and spirolide G (3). While 2 was found to differ from 1 only in that itcontained one less methyl group, 3 was the first spirolide to be isolated that contained a 5:6:6-trispiroketal ring system.The effect of this new feature on the toxicity of 3 relative to other spirolides is presently being pursued.