Impact of Surface Functionalization of AgNPs on Binding and Conformational Change of Hemoglobin (Hb) and Hemolytic Behavior

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• 作者：Loganathan Bhavani Devi ; Sujoy K Das ; Asit Baran Mandal
• 刊名：Journal of Physical Chemistry C
• 出版年：2014
• 出版时间：December 26, 2014
• 年：2014
• 卷：118
• 期：51
• 页码：29739-29749
• 全文大小：536K
• ISSN：1932-7455

文摘

With increasing application of silver nanoparticles (AgNPs) in biological sensing, detection, enzyme immunoassays, and antimicrobial activity as well as SERS, it has been realized that the toxicity limits their application in biomedicine. However, the interaction of proteins with nanoparticle (NPs) surface possessing different chemical functionalities reduces the potential impact of NPs rendering them biocompatible. Here, we have functionalized the AgNPs with 尾-hydroxy propyl cyclodextrin (尾-HPCD-AgNPs) and studied the conformational changes and thermal stability of hemoglobin (Hb) upon interaction with 尾-HPCD-AgNPs. Interaction of Hb with borohydride-capped AgNPs (BH₄-AgNPs) was also evaluated. Hb remained in native form upon binding with 尾-HPCD-AgNPs which is unveiled by UV鈥搗is and fluorescence spectroscopic as well as dynamic light scattering (DLS) studies. However, BH₄-AgNPs induced unfolding of Hb and reduced the 伪-helical content following interaction with Hb. Further, the accessibility of tryptophan fluorescence was found to be 33% for 尾-HPCD-AgNPs quencher, which implies that 尾-HPCD-AgNPs retains the native conformation of Hb Dichroic study revealed that 尾-HPCD-AgNPs did not affect thermal stability of Hb, whereas BH₄-AgNPs decreased the thermal stability of Hb by 5 掳C. Hemolysis assay demonstrated the biocompatibility of 尾-HPCD-AgNPs toward RBC. Functionalization of NPs with controlled surface property thus dictated overall biological reactivity of Hb-AgNPs. Therefore, we trust that the obtained results will help us in designing surface-functionalized AgNPs for biomedical applications.