Detection of Six Genetically Modified Maize Lines Using Optical Thin-Film Biosensor Chips
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- 作者：Sulan Bai ; Jie Zhang ; Shucheng Li ; Haodong Chen ; William Terzaghi ; Xin Zhang ; Xiurong Chi ; Jin Tian ; Hongxia Luo ; Wensheng Huang ; Ying Chen ; Yaochuan Zhang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：August 11, 2010
- 年：2010
- 卷：58
- 期：15
- 页码：8490-8494
- 全文大小：780K
- 年卷期：v.58,no.15(August 11, 2010)
- ISSN：1520-5118
文摘
As more and more genetically modified organisms (GMO) are commercialized, efficient and inexpensive assays are required for their quick detection. An event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. This study developed a system for detecting six GM maize lines (Bt11, Bt176, GA21, MON810, NK603, and T25) using optical silicon thin-film biosensor chips. Aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface. Biotinylated PCR amplicons were then hybridized with the probes. After washing and brief incubation with an anti-biotin IgG horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated PCR amplicons perfectly matched with the probes can be visualized by the color change on the chip surface (gold to blue/purple). This assay is extremely robust, exhibits high sensitivity and specificity, and is flexible from low through moderate to high throughput.