Molecular Structural Differences between Type-2-Diabetic and Healthy Glycogen

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• 作者：Mitchell A. Sullivan ; Jiong Li ; Chuanzhou Li ; Francisco Vilaplana ; David Stapleton ; Angus A. Gray-Weale ; Stirling Bowen ; Ling Zheng ; Robert G. Gilbert
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：June 13, 2011
• 年：2011
• 卷：12
• 期：6
• 页码：1983-1986
• 全文大小：777K
• 年卷期：v.12,no.6(June 13, 2011)
• ISSN：1526-4602

文摘

Glycogen is a highly branched glucose polymer functioning as a glucose buffer in animals. Multiple-detector size exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis were used to examine the structure of undegraded native liver glycogen (both whole and enzymatically debranched) as a function of molecular size, isolated from the livers of healthy and db/db mice (the latter a type 2 diabetic model). Both the fully branched and debranched levels of glycogen structure showed fundamental differences between glycogen from healthy and db/db mice. Healthy glycogen had a greater population of large particles, with more 伪 particles (tightly linked assemblages of smaller 尾 particles) than glycogen from db/db mice. These structural differences suggest a new understanding of type 2 diabetes.