Chemical Changes and Overexpressed Genes in Sweet Basil (Ocimum basilicum L.) upon Methyl Jasmonate Treatment

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• 作者：Zhigang Li ; Xi Wang ; Feng Chen ; Hyun-Jin Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：February 7, 2007
• 年：2007
• 卷：55
• 期：3
• 页码：706 - 713
• 全文大小：299K
• 年卷期：v.55,no.3(February 7, 2007)
• ISSN：1520-5118

文摘

The effects of methyl jasmonate (MeJA) on the production of bioactive chemicals and gene expressionin sweet basil were investigated. The total amount of phenolic compounds significantly increased insweet basil after 0.5 mM MeJA treatment. Among the phenolic compounds, rosmarinic acid (RA)and caffeic acid (CA) were identified, and their amounts increased by 55 and 300%, respectively.The total amount of terpenoids also significantly increased after the same treatment. Particularly,eugenol and linalool increased by 56 and 43%, respectively. To better understand the signaling effectof MeJA on sweet basil, suppression subtractive hybridization (SSH) was used to identify the MeJAup-regulated genes. Among the 576 cDNA clones screened from the forward SSH cDNA library, 28were found to be up-regulated by the MeJA treatment. Sequencing of these cDNA clones followedby BLAST searching revealed six unique transcripts displaying high similarities to the known enzymesand peptide, that is, lipoxygenase (LOX), cinnamic acid 4-hydroxylase (C4H), prephenate dehydrogenase (PDH), polyphenol oxidase (PPO), acid phosphatase (APase), and pentatricopeptide repeat(PPR), which play significant roles in the formation of secondary metabolites in sweet basil. Northernblot further confirmed the increased production at transcriptional level of LOX, C4H, PDH, PPO, PPR,and APase.