Re-Electrospraying Splash-Landed Proteins and Nanoparticles
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- 作者：W. Henry Benner ; Gregory S. Lewis ; Susanne V. Hering ; Brent Selgelke ; Michelle Corzett ; James E. Evans ; Felice C. Lightstone
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：March 6, 2012
- 年：2012
- 卷：84
- 期：5
- 页码：2498-2504
- 全文大小：327K
- 年卷期：v.84,no.5(March 6, 2012)
- ISSN：1520-6882
文摘
FITC-albumin, Lsr-F, or fluorescent polystyrene latex particles were electrosprayed from aqueous buffer and subjected to dispersion by differential electrical mobility at atmospheric pressure. A resulting narrow size cut of singly charged molecular ions or particles was passed through a condensation growth tube collector to create a flow stream of small water droplets, each carrying a single ion or particle. The droplets were splash landed (impacted) onto a solid or liquid temperature controlled surface. Small pools of droplets containing size-selected particles, FITC-albumin, or Lsr-F were recovered, re-electrosprayed, and, when analyzed a second time by differential electrical mobility, showed increased homogeneity. Transmission electron microscopy (TEM) analysis of the size-selected Lsr-F sample corroborated the mobility observation.