Sol鈥揋el-Based Titania鈥揝ilica Thin Film Overlay for Long Period Fiber Grating-Based Biosensors

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• 作者：Francesco Chiavaioli ; Palas Biswas ; Cosimo Trono ; Sunirmal Jana ; Somnath Bandyopadhyay ; Nandini Basumallick ; Ambra Giannetti ; Sara Tombelli ; Susanta Bera ; Aparajita Mallick ; Francesco Baldini
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：December 15, 2015
• 年：2015
• 卷：87
• 期：24
• 页码：12024-12031
• 全文大小：451K
• ISSN：1520-6882

文摘

An evanescent wave optical fiber biosensor based on titania鈥搒ilica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol鈥揼el-based titania鈥搒ilica thin film, deposited along the sensing portion of the fiber by means of the dip-coating technique. Changing both the sol viscosity and the withdrawal speed during the dip-coating made it possible to adjust the thickness of the film overlay, which is a crucial parameter for the sensor performance. After the functionalization of the fiber surface using a methacrylic acid/methacrylate copolymer, an antibody/antigen (IgG/anti-IgG) assay was carried out to assess the performance of sol鈥揼el based titania鈥搒ilica-coated LPGs as biosensors. The analyte concentration was determined from the wavelength shift at the end of the binding process and from the initial binding rate. This is the first time that a sol鈥揼el based titania鈥搒ilica-coated LPG is proposed as an effective and feasible label-free biosensor. The specificity of the sensor was validated by performing the same model assay after spiking anti-IgG into human serum. With this structured LPG, detection limits of the order of tens of micrograms per liter (10^鈥?1 M) are attained.