Nanoscale E-Cadherin Ligand Patterns Show Threshold Size for Cellular Adhesion and Adherence Junction Formation

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• 作者：Stine H. Kristensen ; Gitte A. Pedersen ; Lene N. Nejsum ; Duncan S. Sutherland
• 刊名：Nano Letters
• 出版年：2012
• 出版时间：April 11, 2012
• 年：2012
• 卷：12
• 期：4
• 页码：2129-2133
• 全文大小：280K
• 年卷期：v.12,no.4(April 11, 2012)
• ISSN：1530-6992

文摘

The role of ligand spatial distribution on the formation of cadherin mediated cell鈥揷ell contacts is studied utilizing nanopatterns of E-cadherin ligands. Protein patches ranging in size from 100 to 800 nm prepared by colloidal lithography critically influence adhesion, spreading, and formation of adherence junctions in epithelial cells. Cells at 100 nm patterns show poor adhesion, while larger pattern sizes show good adhesion, significant spreading, and defined cortical actin. We estimate a threshold of 0.03 渭m² for epithelial cellular attachment via E-Cadherin.

Keywords:

Protein nanopatterns; adherens junctions; E-cadherin; cell adhesion; sparse colloidal lithography