Conjugation of Biomolecules with Luminophore-Doped Silica Nanoparticles for Photostable Biomarkers
详细信息 查看全文
- 作者：Swadeshmukul Santra ; Peng Zhang ; Kemin Wang ; Rovelyn Tapec ; and Weihong Tan
- 刊名：Analytical Chemistry
- 出版年：2001
- 出版时间：October 15, 2001
- 年：2001
- 卷：73
- 期：20
- 页码：4988 - 4993
- 全文大小：123K
- 年卷期：v.73,no.20(October 15, 2001)
- ISSN：1520-6882
文摘
A new molecular conjugation method has been developedto label biomolecules with optically stable metalorganicluminophores, such as tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), which are otherwise notpossible for direct linking with the biomolecules. Uniquebiochemical properties of the biomolecule can, thus, beassociated with photostable luminophores. This opens ageneral way to conjugate desired biomolecules using asensitive signal transduction method. It also promotes theapplication of excellent luminescent materials, especiallythose based on photostable metalorganic luminophores,in biochemical analysis and biomolecular interactionstudies. The conjugation method is based on uniformluminophore-doped silica (LDS) nanoparticles (63 ± 4nm). These nanoparticles have been prepared using awater-in-oil (W/O) microemulsion method. The controlledhydrolysis of tetraethyl orthosilicate (TEOS) in W/Omicroemulsion leads to the formation of monodisperseLDS nanoparticles. The luminophores are doped insidethe nanoparticles, and the particle's silica surfaces canbe used to covalently bind with biomolecules. The luminophores are well-protected from the environmentaloxygen when they are doped inside the silica network. Asan example, we used an antibody for leukemia cellrecognition. The antibody was first immobilized onto theluminophore-doped nanoparticle through silica chemistryand then was used for leukemia cell identification by anoptical microscopy imaging technique. The leukemia cellswere identified easily, clearly, and with high efficiencyusing these antibody-coated nanoparticles. The advantages of using small, uniform luminophore-doped nanoparticles are discussed.