Characterization of a Vitamin B12 Compound from Unicellular Coccolithophorid Alga (Pleurochrysis carterae)
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- 作者：Emi Miyamoto ; Fumio Watanabe ; Syuhei Ebara ; Shigeo Takenaka ; Hiroyuki Takenaka ; Yuji Yamaguchi ; Norihide Tanaka ; Hiroshi Inui ; and Yoshihisa Nakano
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2001
- 出版时间：July 2001
- 年：2001
- 卷：49
- 期：7
- 页码：3486 - 3489
- 全文大小：58K
- 年卷期：v.49,no.7(July 2001)
- ISSN：1520-5118
文摘
A unicellular coccolithophorid alga, Pleurochrysis carterae, contained 125.4 ± 1.2 
g of vitamin B12per 100 g dry cell weight of the lyophilized algal cells. A vitamin B12 compound was purified fromthe lyophilized algal cells and partially characterized. The silica gel 60 TLC and reversed-phaseHPLC patterns of the purified pink-colored compound were identical to those of authentic vitaminB12, but not those of vitamin B12 analogues inactive for humans. When 22-week-old B12-deficientrats which excreted substantial amounts of methylmalonic acid (75.5 ± 12.3 mg/day) in urine werefed the P. carterae (10 g per kg diet)-supplemented diet for 12 d, urinary methylmalonic acid excretion(as an index of vitamin B12 deficiency) of the rats became undetectable and hepatic vitamin B12level of the rats was significantly increased.Keywords: Coccolithophorid alga; Lactobacillus delbruekii; intrinsic factor; Pleurochrysis carterae;vitamin B12
g of vitamin B12per 100 g dry cell weight of the lyophilized algal cells. A vitamin B12 compound was purified fromthe lyophilized algal cells and partially characterized. The silica gel 60 TLC and reversed-phaseHPLC patterns of the purified pink-colored compound were identical to those of authentic vitaminB12, but not those of vitamin B12 analogues inactive for humans. When 22-week-old B12-deficientrats which excreted substantial amounts of methylmalonic acid (75.5 ± 12.3 mg/day) in urine werefed the P. carterae (10 g per kg diet)-supplemented diet for 12 d, urinary methylmalonic acid excretion(as an index of vitamin B12 deficiency) of the rats became undetectable and hepatic vitamin B12level of the rats was significantly increased.Keywords: Coccolithophorid alga; Lactobacillus delbruekii; intrinsic factor; Pleurochrysis carterae;vitamin B12