Layer-by-Layer Self-Assembled Osmium Polymer-Mediated Laccase Oxygen Cathodes for Biofuel Cells: The Role of Hydrogen Peroxide
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- 作者：Pablo Scodeller ; Romina Carballo ; Rafael Szamocki ; Laura Levin ; Flavia Forchiassin ; Ernesto J. Calvo
- 刊名：Journal of the American Chemical Society
- 出版年：2010
- 出版时间：August 18, 2010
- 年：2010
- 卷：132
- 期：32
- 页码：11132-11140
- 全文大小：297K
- 年卷期：v.132,no.32(August 18, 2010)
- ISSN：1520-5126
文摘
High potential purified Trametes trogii laccase has been studied as a biocatalyst for oxygen cathodes composed of layer-by-layer self-assembled thin films by sequential immersion of mercaptopropane sulfonate-modified Au electrode surfaces in solutions containing laccase and osmium-complex bound to poly(allylamine), (PAH−Os). The polycation backbone carries the Os redox relay, and the polyanion is the enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2 reduction, with scanning electrochemical microscopy (SECM). Furthermore the inhibitory effect of peroxide on the biocatalytic current resulted in abnormal current dependence on the O2 partial pressure and peak shape with hysteresis in the polarization curves under stagnant conditions, which is offset upon stirring with the RDE. The new kinetic evidence reported in the present work is very relevant for the operation of biofuel cells under stagnant conditions of O2 mass transport.