Lanthanide-Chelating Carbohydrate Conjugates Are Useful Tools To Characterize Carbohydrate Conformation in Solution and Sensitive Sensors to Detect Carbohydrate鈥揚rotein Interactions

详细信息    查看全文

• 作者：脕ngeles Canales ; 脕lvaro Mallagaray ; M. 脕lvaro Berb铆s ; Armando Navarro-V谩zquez ; Gema Dom铆nguez ; F. Javier Ca帽ada ; Sabine Andr茅 ; Hans-Joachim Gabius ; Javier P茅rez-Castells ; Jes煤s Jim茅nez-Barbero
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：June 4, 2014
• 年：2014
• 卷：136
• 期：22
• 页码：8011-8017
• 全文大小：403K
• 年卷期：v.136,no.22(June 4, 2014)
• ISSN：1520-5126

文摘

The increasing interest in the functional versatility of glycan epitopes in cellular glycoconjugates calls for developing sensitive methods to define carbohydrate conformation in solution and to characterize protein鈥揷arbohydrate interactions. Measurements of pseudocontact shifts in the presence of a paramagnetic cation can provide such information. In this work, the energetically privileged conformation of a disaccharide (lactose as test case) was experimentally inferred by using a synthetic carbohydrate conjugate bearing a lanthanide binding tag. In addition, the binding of lactose to a biomedically relevant receptor (the human adhesion/growth-regulatory lectin galectin-3) and its consequences in structural terms were defined, using Dy³⁺, Tb³⁺, and Tm³⁺. The described approach, complementing the previously tested protein tagging as a way to exploit paramagnetism, enables to detect binding, even weak interactions, and to characterize in detail topological aspects useful for physiological ligands and mimetics in drug design.