Dosimetry of N6-Formyllysine Adducts Following [13C2H2]-Formaldehyde Exposures in Rats

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• 作者：Bahar Edrissi ; Koli Taghizadeh ; Benjamin C. Moeller ; Dean Kracko ; Melanie Doyle-Eisele ; James A. Swenberg ; Peter C. Dedon
• 刊名：Chemical Research in Toxicology
• 出版年：2013
• 出版时间：October 21, 2013
• 年：2013
• 卷：26
• 期：10
• 页码：1421-1423
• 全文大小：194K
• 年卷期：v.26,no.10(October 21, 2013)
• ISSN：1520-5010

文摘

With formaldehyde as the major source of endogenous N⁶-formyllysine protein adducts, we quantified endogenous and exogenous N⁶-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [¹³C²H₂]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N⁶-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N⁶-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts.