Influence of Hydrophobic Structures on the Plasma Membrane Permeability of Lipidlike Molecules

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• 作者：Kenichi Niikura ; Katsuyuki Nambara ; Takaharu Okajima ; Yasutaka Matsuo ; Kuniharu Ijiro
• 刊名：Langmuir
• 出版年：2010
• 出版时间：June 15, 2010
• 年：2010
• 卷：26
• 期：12
• 页码：9170-9175
• 全文大小：887K
• 年卷期：v.26,no.12(June 15, 2010)
• ISSN：1520-5827

文摘

A series of FITC-labeled hydrophobic molecules (1−8) were prepared, and their cellular uptakes have been investigated using cell-cycle-synchronized HeLa cells. The cellular membrane permeability of compounds strongly depended on both the chemical structure and the cell-cycle phase. In the G1/S phase, branched hydrocarbon-containing 3 and cis-olefin-containing 2 and 8 were efficiently internalized into cells by passive diffusion. In contrast, linear alkyl chain-containing 1 and 7 were retained on the membrane without rapid internalization. In the M phase, rapid permeation was suppressed for all molecules.