A Two-Stage One-Pot Enzymatic Synthesis of TDP-L-mycarose from Thymidine and Glucose-1-phosphate

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• 作者：Haruko Takahashi ; Yung-nan Liu ; and Hung-wen Liu
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：February 8, 2006
• 年：2006
• 卷：128
• 期：5
• 页码：1432 - 1433
• 全文大小：57K
• 年卷期：v.128,no.5(February 8, 2006)
• ISSN：1520-5126

文摘

This report describes a procedure combining six enzymes native to Escherichia coli or Salmonella typhi, such as thymidine kinase (TK), thymidylate kinase (TMK), nucleoside diphosphate kinase (NDK), pyruvate kinase (PK; for ATP regeneration), TDP-glucose synthetase (RfbA), and TDP-glucose 4,6-dehydratase (RfbB), with five enzymes from Streptomyces fradiae, such as TylX3, TylC1, TylC3, TylK, and TylC2, that resulted in the biosynthesis of TDP-L-mycarose from glucose-1-phosphate and thymidine. This two-stage one-pot approach can be readily applied to the synthesis of other unusual sugars.