Effects of Salt Concentrations of the Aqueous Peptide-Amphiphile Solutions on the Sol鈥揋el Transitions, the Gelation Speed, and the Gel Characteristics
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- 作者：Takahiro Otsuka ; Tomoki Maeda ; Atsushi Hotta
- 刊名：Journal of Physical Chemistry B
- 出版年：2014
- 出版时间：October 2, 2014
- 年：2014
- 卷：118
- 期：39
- 页码：11537-11545
- 全文大小：493K
- ISSN：1520-5207
文摘
Hydrogels made of peptide amphiphiles (PA) have attracted a lot of interest in biomedical fields. Considering the applications of PA hydrogels, the control of the gelation speed and the gel characteristics is essential to predominantly determine the usefulness and practicability of the hydrogels. In this work, the effects of the salt concentrations using sodium dihydrogenorthophosphate (NaH2PO4) on the sol鈥揼el transition behaviors, especially the gelation speed and the gel characteristics of the designed PA (C16鈥揥3K) hydrogels in aqueous solution were discussed. It was found that the original solution state before rheological testing was independent of the salt concentration, which was confirmed by observing the self-assembly structures and the peptide secondary structures of PA through transmission electron microscopy (TEM) and circular dichroism spectroscopy (CD). The PA solutions with different salt concentrations, however, presented a profound difference in the gelation speed and the gel characteristics: the solution exhibited higher gelation speeds and higher mechanical properties at higher salt concentrations. Concurrently, the density, the length of wormlike micelles, and the conformational ratio of 尾-sheets to 伪-helices in the equilibrium PA solutions all increased with the increase in the salt concentrations.