Synthesis, Characterization, and Application of Eu(III), Tb(III), Sm(III), and Dy(III) Lanthanide Chelate Nanoparticle Labels

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• 作者：Petri Huhtinen ; Mirja Kivelä ; Outi Kuronen ; Virve Hagren ; Harri Takalo ; Heikki Tenhu ; Timo Lö ; vgren ; and Harri Hä ; rmä
• 刊名：Analytical Chemistry
• 出版年：2005
• 出版时间：April 15, 2005
• 年：2005
• 卷：77
• 期：8
• 页码：2643 - 2648
• 全文大小：123K
• 年卷期：v.77,no.8(April 15, 2005)
• ISSN：1520-6882

文摘

Preparation and characterization of europium(III), terbium(III), samarium(III), and dysprosium(III) polystyrene nanoparticle labels with lanthanide-specific fluorescence properties has been presented. Emulsion copolymerization of styrene and acrylic acid was used to synthesize uniform-sized nanoparticles ~45 nm in diameter.Europium(III) and samarium(III) lanthanides were chelated with 2-naphthoyltrifluoroacetone and trioctylphosphine oxide to dye the spherical particles, whereasterbium(III) and dysprosium(III) chelate complexes contained a newly synthesized ligand, 4-(2,4,6-tridecyloxyphenyl)pyridine-2,6-dicarboxylic acid. The fluorescenceproperties of the four lanthanides-including a wideStokes shift, a narrow emission peak, and long fluorescence lifetime-were retained despite the incorporationinto the nanoparticles. Furthermore, the nanoparticles,containing more than 1000 lanthanide chelates, weredetectable at label concentrations 3 orders of magnitudelower than the corresponding soluble lanthanide chelatelabels. The applicability of the labels prepared was demonstrated by a heterogeneous sandwich-type immunoassay for human prostate-specific antigen, where the lowestlimits of detection of 1.6, 2.4, 10.1, and 114.2 ng/L wereachieved using europium(III), terbium(III), samarium(III), and dysprosium(III) nanoparticles, respectively. Thespectral and functional properties of the lanthanide-embedded polystyrene nanoparticles developed here suggest that the technology is applicable for high-sensitivitymulticolor assays.